Order Histology Services

Order Histology Services

Download and complete an order form below, and send a printed version with the samples. If you have any questions, please call us on 21 62 49 80 or email post.fvgno@fishvetgroup.com and we will be able to help.

order-form-histology

When samples and order forms arrive at our laboratory, a confirmation will be sent to you by email.

This is when our automatic tracking of your samples begins. This is part of our quality assurance and contribute to high quality sample handling and traceability during analysis.

 Order Sampling Equipment

To order our sampling equipment please contact us via:

Histology jars are available with or without formalin and come in boxes of 50 jars.

We also offer racks with 32, 64 and 88 tubes.

The equipment is sent with normal postal service unless otherwise agreed upon. Delivery normally takes about 4-7 working days.

Order Sampling Equipment Now

Your Name (required)

Your Company Name (required)

Your Email (required)

Phone Number (required)

Quantity of PCR 32:
Quantity of PCR 64:
Quantity of PCR 88:
Histo Jars with Formalin (Quantity of 50 jar boxes):
Histo Jars without Formalin (Quantity of 50 jar boxes) :

If you would like to request other numbers of tubes, please enter it into the ‘Other information’ field

Delivery Address

Other information

Fish Vet Group Norge uses barcoded sample containers. These codes are linked to you as a customer. Upon receipt of specimens and requisition form, begins our automated tracking of your tests and you will receive confirmation of receipt by email. Use of barcodes and automatic tracking is part of our quality assurance and are used to ensure the quality of the sample handling and traceability in analytical work.

How to take a Histology sample

Guide for sampling and shipping for histopathological evaluation

Organ set

  • For histopathology, we recommend sampling from a minimum of 5 fish.
  • Organs from each individual should be placed in separate formalin containers.
  • Label each container with fish identity and mark on order form which samples are from each individual.
  • From each individual, we recommend that the following tissues are sampled: pyloric caeca with pancreas, gills, heart, skin/muscle, liver, spleen, kidney. From marine fish species, eye and brain should be included.
  • In case of central nervous symptoms, suspicion of VHS or nodavirus, brain should be included.

Single organs from several individuals

  • Tissues from several individuals can be placed in the same formalin container.

In general

  • The volume of formalin should be at least 10 times the total volume of tissue.
  • Use leak-proof containers of sufficient size, enabling tissues to float freely. Containers can be ordered from Fish Vet Group Norge.
  • Cut out slices of tissue (0,4 cm x 1 cm x 1 cm) with a flat cut surface where possible. Avoid squeezing tissues when handling. When submitting samples for urgent analysis, it is especially important that tissues slices are not too thick as skin and muscle samples may not fix sufficiently.
  • In case of gross lesions, the sample should be taken from the transition between healthy and affected tissue.
  • If tissue is mechanically damaged from euthanasia or the fish is small (< 4 cm), euthanasia should be carried out using an overdose of anesthetic.
  • Larvae and juveniles should be euthanized with anesthetic prior to fixation
    • Larvae/juveniles < 5 mm: fixated whole
    • Larvae/juveniles > 5 mm: remove operculum on one side and gently open abdomen along ventrally to allow formalin to permeate.
Pyloric caeca with pancreas Tissue slices (max 0,4x1x1 cm)
Gills Second gill arch (larger fish: 2-3 cm of gill arch below angle)

Gill tissue is very prone to autolysis, hence tissue should be put immediately on formalin. To ensure rapid fixation of whole tissue, container should be turned or shaken gently immediately to avoid lamellae sticking together.

Brain For brain histology, fish should be euthanized with overdose of anesthetic. For small fish, head is cleaved, lateral to the midline. Fix the larger half of the brain inside skull. For larger fish, brain can be dissected out and fixed whole.
Heart Whole organ (atrium, ventricle and bulbus arteriosus). For large fish: place the heart with the apex towards you and bulbus away from you and cut vertically though atrium and ventricle.
Skin/muscle Take out a vertical slice across the lateral line at the level of the dorsal fin, including both red and white muscle
Liver Tissue slices (max 0,4x1x1 cm)
Spleen Tissue slices (max 0,4x1x1 cm)
Kidney Fix the whole organ. For larger fish: cut out a section across the middle kidney including the renal capsule.
Eye Fix whole, preferably on 10 % unbuffered formalin.

 

How to send Histology Samples

If you have an existing agreement with an agreement number or project number, please add it to the relevant field in the form.

Courier:
Fish Vet Group Norge
Hoffsveien 21-23
0275 OSLO
Norway

Post:
Fish Vet Group Norge
Postboks 1012
0218 OSLO
Norway

Recommended submission method for histology samples:

  • 24 hour delivery – Express – Overnight
  • 3-day delivery – Business Package or faster
  • 6-day delivery – Business Package

Regular mail is also an option.

Packaging and shipping guidelines

Samples should be shipped as soon as possible after sampling.

Samples should be kept cool, but not frozen until shipment. Freezing hampers histopathological examination.

Avoid spills on the packaging / requisition form.

Please label all samples clearly and unambiguously. The labeling should also be indicated on the requisition form.

All samples should be packed in three layers of packaging:

  • One or more leak-proof primary receptacles (formalin glass). Use airtight container large enough for the tissue pieces to move freely in the fixative.
  • A leak-proof secondary container (especially important for liquid samples)
  • A sufficiently strong outer packaging (padded envelope, stiff cardboard, polystyrene etc.)
  • For liquid samples please include plenty of absorbent material between the primary and secondary packaging
  • See also Post Office regulations for the shipment of biological materials.
  • Please label the package: “BIOLOGICAL SUBSTANCE CATEGORY B”

Delivery of Histology results

  • 6 working days delivery – The report will be delivered 6 working days after we have received your sample.
  • 3 working days delivery – The report will be delivered 3 working days after we have received your sample. This service entails a 100% additional cost and should be agreed in advance to ensure relevant personnel is available.
  • 24 hour delivery (Emergency service) – The report will be delivered 24 hours after we have received your sample. This service entails a 400% surcharge and should be agreed in advance to ensure relevant personnel is available.

An agreement must be in place for shipments with a number of samples more than the below for the following reporting times:

  • 3 and 6 working day delivery
    • Organ package of up to 20 fish
    • Up to 30 gills/individual organs
  • 24 hour delivery (Emergency service), (should always be pre-arranged)
    • Normally an organ packages of up to 10 fish or 20 gill preparations / single organs.

For analyses with systematic evaluation of tissue/organs beyond common disease diagnostics are considered projects and should be agreed with us in advance.