Order Bacteriology

Order bacteriology services

Download and complete an order form below, and send a printed version with the samples. If you have any questions, please call us on 21 62 49 80 or email post.fvgno@fishvetgroup.com and we will be able to help.

order-form-bacteriology

For normal post, the address is:

Fish Vet Group Norge
Postboks 1012
0218 Oslo

For courier delivery, the address is:

Fish Vet Group Norge
Hoffsveien 21-23
0275 Oslo

When samples and order forms arrive at our laboratory, a confirmation will be sent to you by email.

This is when our automatic tracking of your samples begins. This is part of our quality assurance and contribute to high quality sample handling and traceability during analysis.

How to take a Bacteriology sample

The following procedure should be followed to ensure good quality of samples for bacterial identification.

Recommended equipment for sampling:

  • Agar plates: blood plate and blood plate with NaCl. If Flavobacterium sp. is suspected, samples should in addition be seeded out on Ordal plates. If Tenacibaculum sp. is suspected, samples should in addition be seeded out on marine agar. Contact us (post.fvgno@fishvetgroup.com) for information on suppliers of agar plates.
  • Swabs: Transsystem Amies charcoal is recommended. Contact us (post.fvgno@fishvetgroup.com) for more information on suppliers of swabs.
  • Platinum inoculation loop and disposable plastic inoculation loops
  • Scissors and forceps
  • Scalpel blades and scalpel blade holder
  • Flame burner for sterilizing instruments
  • Bench paper or disposable paper plate as dissecting surface
  • Disposable gloves
  • Water and alcohol resistant marker pen
  • 70% ethanol for disinfection
  • Frozen ice packs
  • Suitable material for wrapping and posting samples

Recommended procedure for sampling:

  •  Preparation of agar plates:
    • Remove condensation inside lid by hitting lid against flat, clean tissue
    • Label plates clearly with water and alcohol resistant pen
    • Agar plates/swabs/organs and whole fish should be labelled with ID, date and organ/tissue from whish the sample is taken
    • Fill in the same labelling in the order form
  • Kidney:
    • Place fish on side with head facing left
    • Remove the body wall using a sterile scalpel blade or scissors
    • Pull abdominal organs away from swim bladder. Remove swim bladder to access kidney
    • Puncture the head kidney with a glowing platinum inoculation loop which is inserted a short distance. Allow loop to cool before inserting it a little further. Retract gently avoiding contamination of loop
    • Inoculate agar plate
  •  Spleen:
    • Dissect fish as described under “kidney”
    • Puncture splenic capsule with a glowing inoculation loop with is inserted a short distance. Allow loop to cool before inserting it a little further. Gently retract avoiding contamination of loop
    • Inoculate agar plate
  •  Skin ulcer
    • Insert a sterile inoculation loop under the skin at the wound edge
    • Gently retract and inoculate agar plate
  •  Small fish or spawn
    • Hold fish with forceps with the back pointing upwards
    • Cut of head with sterile scissors or scalpel to expose head kidney
    • For small fish: Insert a sterile cooled inoculation loop a short distance into head kidney. Retract gently and inoculate agar plate
    • For spawn: Squeeze body while the cut surface is pressed gently on to an agar plate
  • Gill:
    • Remove operculum with sterile scissors
    • With sterile scissors, cut out the whole second gill arch (small fish) or a 2 cm long segment of this (large fish)
    • Press gill gently down on to an agar plate. Remove gill and make dilutions from the imprint
  • Intestine:
    • Sterilize vent using 70% ethanol
    • Insert sterile swab into hindgut
    • Alternatively, dissect as described above, open intestine and insert a sterile inoculation loop into intestinal content
    • Inoculate agar plate

 

Recommended procedure for streaking out an agar plate

For optimal dilution of bacterial colonies it is recommended that each sample is seeded out on a separate agar plate. If kidney and skin ulcer is sampled from the same fish, these samples can be seeded out on the same plate by splitting the plate into two with a marker pen at bottom of plate

  • Dilutions should be performed as demonstrated on Figure 1. It is recommended to change inoculation loop between each dilution.
figure-1

Figure 1. Correct technique for seeding out an agar plate.

 

How to send Bacteriology Samples

If you have an existing agreement with an agreement number or project number, please add it to the relevant field in the form.

By Courier

Fish Vet Group Norge
Hoffsveien 21-23
0275 OSLO
Norway

By Post

Fish Vet Group Norge
Postboks 1012
0218 OSLO
Norway

Recommended method of sending bacteriology samples are business package (courier).

We also recommend:

  • Agar plates must be sealed with parafilm or similar, then packed in leak-proof secondary container surrounded by a layer absorbent paper. Secondary container should be packed in a sufficiently strong, insulating outer packaging (eg. polystyrene box) filled with ice packs.
  • Swabs should be placed in a leak-proof secondary container (eg. sealed plastic bag) and wrapped in absorbent paper. It shall then be placed in a sufficiently strong insulating outer packaging (eg. padded envelope) filled with ice packs.
  • Whole fish less than 1 kg should be packed individually in absorbent paper and double plastic bags, then in leak-proof secondary container and sufficiently strong, insulating outer packaging (eg. polystyrene box) filled with ice or ice packs. Generally it is recommended to submit freshly streaked agar plates, rather than whole fish, especially for investigating wounds in the skin.

Delivery of Bacteriology results

Delivery time is dependent on the bacterial growth rate. We strive for a short delivery time and report will be issued as soon as analysis goods available.